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[目的]研究miR-34b/c启动子区单核苷酸多态rs4938723(T/C)对其转录调控的影响。[方法]构建含有不同基因型miR-34b/c启动子区的报告基因表达载体pGL3-basic-miR34b/c-T和pGL3-basic-miR34b/c-C,分别与内参质粒SV40共转染于Hela、A549和CHO三种细胞后进行荧光素酶活性分析。[结果]pGL3-basic-miR34b/c-C等位基因组荧光素酶相对活性与pGL3-promoter-miR30C-T组等位基因相比,均有显著升高(P<0.01)。[结论]荧光素酶活性强弱间接反映了miRNA启动子区遗传变异对转录活性的影响,当miR-34b/c启动子区rs4938723位点为C时,可以更为有效地增加miR-34b/c的转录产物,从而在转录水平上调控miRNA的表达。
[Objective] To investigate the effect of single nucleotide polymorphism rs4938723 (T / C) on the transcriptional regulation of miR-34b / c promoter. [Methods] The reporter gene expression vectors pGL3-basic-miR34b / cT and pGL3-basic-miR34b / cC containing different genotypes of miR-34b / c promoter region were constructed and transfected into Hela, A549 and After three kinds of CHO cell luciferase activity analysis. [Result] The relative luciferase activity of pGL3-basic-miR34b / c-C allele group was significantly higher than that of pGL3-promoter-miR30C-T group (P <0.01). [Conclusion] The luciferase activity indirectly reflects the effect of genetic variation of miRNA promoter on transcriptional activity. When rs4938723 of miR-34b / c promoter is C, miR-34b / c transcripts, thereby regulating the expression of miRNAs at the transcriptional level.