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以“津优35”黄瓜为试材,研究了日光温室地下滴灌条件下水氮耦合对黄瓜植株氮代谢过程中关键酶的影响。结果表明:灌水量和施N量对日光温室地下滴灌条件下黄瓜植株氮代谢酶活性均有显著影响。在同一施氮水平下,硝酸还原酶(NR)、谷氨酰胺合成酶(GS)活性随灌水量的增加而显著升高,而谷氨酸合成酶(GOGAT)活性在I2(0.8Ep)、I3(0.8Ep)2个灌溉水平下无显著差异;结果期黄瓜植物叶片中硝态N含量、可溶性蛋白质含量随灌水量的增加而降低,但I2、I3处理差异不显著。在I2、I3灌溉水平下,N2(600kg/hm2)处理下硝酸还原酶、谷氨酰胺合成酶、谷氨酸脱氢酶(glutamate dehydrogenase GDH)活性显著高于N3处理,而谷氨酸合成酶活性、硝态氮含量、可溶性蛋白质含量与N3(750kg/hm2)处理无显著差异。根据黄瓜氮代谢相关酶及相关物质含量,日光温室地下滴灌条件下黄瓜作物最佳灌水量应为0.8Ep,施N量为600kg/hm2。
The effect of water and nitrogen coupling on the key enzymes in the process of nitrogen metabolism in cucumber plants was studied using “Jinyou35 ” cucumber as test material. The results showed that irrigation amount and N application rate had significant effects on the activities of nitrogen metabolism enzymes in cucumber under the condition of subsurface drip irrigation in solar greenhouse. Nitrate reductase (NR) and glutamine synthetase (GS) activity increased significantly with the increase of irrigation rate under the same nitrogen application level, while glutamate synthetase (GOGAT) activity increased at I2 (0.8Ep), I3 (0.8Ep). There was no significant difference in nitrate content and soluble protein content between the cucumber plants and the control plants. Nitrate reductase, glutamine synthetase and glutamate dehydrogenase (GDH) activities were significantly higher under N 2 (600 kg / hm 2) treatment than those under N 3 treatment at I2 and I 3 irrigation levels, whereas glutamate synthase There was no significant difference in activity, nitrate content, soluble protein content and N3 (750kg / hm2) treatment. According to the contents of enzymes and related substances related to nitrogen metabolism in cucumber, the optimum irrigation amount of cucumber crops under the greenhouse irrigation was 0.8Ep and the N application rate was 600kg / hm2.