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目的研究乙肝病毒DNA含量与乙肝病毒感染免疫学标志物是否具有相关性。方法应用荧光定量PCR法对500例乙型肝炎患者及100例健康对照者的血清进行HBVDNA含量检测,并用ELISA法进行乙型肝炎病毒免疫学标志物检测。结果不同组之间DNA含量有差异(P<0.01)。HBsAg(+),HBeAb(+),HBcAb(+)组患者血清HBVDNA含量阳性检出率明显高于除HBsAg(+)、HBeAb(+)、HBcAb-IgM(+)组以外的其他各组(P<0.01)。HBeAg阳性和HBVDNA阳性有较高的一致性(P<0.01)。结论 500例乙型肝炎患者中HBsAg(+),HBeAb(+),HBcAb(+)组患者血清HBVDNA含量阳性检出率及HBVDNA的平均含量最高,HBeAg检测和DNA定量检测阳性率具有较高一致性。
Objective To study whether the DNA content of hepatitis B virus is related to immunological markers of hepatitis B virus infection. Methods The serum of HBVDNA in 500 hepatitis B patients and 100 healthy controls were detected by real-time quantitative PCR, and the hepatitis B virus immunological markers were detected by ELISA. Results There was difference in DNA content between different groups (P <0.01). The positive rate of HBVDNA in HBsAg (+), HBeAb (+) and HBcAb (+) patients was significantly higher than that in HBsAg (+), HBeAb (+) and HBcAb-IgM P <0.01). HBeAg-positive and HBVDNA-positive have a higher consistency (P <0.01). Conclusion The positive rate of HBVDNA and the average content of HBVDNA in HBsAg (+), HBeAb (+) and HBcAb (+) patients of 500 hepatitis B patients were the highest, and the positive rates of HBeAg and DNA were the highest Sex.