论文部分内容阅读
目的:探讨糖肾平胶囊对糖尿病肾病大鼠肾脏保护作用及其对肾组织单核细胞趋化因子(MCP-1)蛋白及mRNA表达的影响。方法:雄性SD大鼠,50只,按体重均匀分出正常组10只,其余40只,腹腔注射链脲佐菌素造模。将造模成功的大鼠按血糖水平分为模型组、厄贝沙坦组、糖肾平胶囊组,用药12周。每周称量体重;4,8,12周后分笼收集24 h尿液检测24 h尿蛋白定量,处死大鼠行血液生化指标BUN、Scr、TG检测,肾组织行HE、Mallory染色,观察病理形态学改变,采用免疫组化、原位杂交分析肾组织MCP-1蛋白及mRNA的表达。结果:模型组大鼠摄食减少、精神不振、活动迟缓、尿量减少、体重减轻以及肾组织病理改变,而厄贝沙坦组和糖肾平胶囊组均有不同程度的改善,且糖肾平胶囊组优于厄贝沙坦组;正常组肾小管上皮细胞、肾间质细胞胞浆有少量MCP-1蛋白及mRNA表达;模型组则呈强阳性表达。糖肾平胶囊组MCP-1蛋白及mRNA的表达较模型组明显减少(P<0.05)。结论:糖肾平胶囊可能通过对肾组织MCP-1蛋白及mRNA表达下调而发挥治疗和预防糖尿病肾病的作用。
Objective: To investigate the protective effects of Tangshenping Capsule on diabetic nephropathy in rats and its effects on the expression of monocyte chemotactic factor (MCP-1) and mRNA. Methods: Fifty male SD rats were randomly divided into normal group (n = 10) according to body weight. The remaining 40 rabbits were injected intraperitoneally with streptozotocin. The successful model rats were divided into model group, irbesartan group and Tangshenping capsule group by blood glucose level for 12 weeks. Body weight was weighed every week. Urine was collected 24 hours after 4, 8, and 12 weeks to detect urinary protein in 24 hours. Blood samples were collected for determination of BUN, Scr and TG. Renal tissues were stained with HE and Mallory. Pathological changes were observed by immunohistochemistry, in situ hybridization analysis of renal MCP-1 protein and mRNA expression. Results: The rats in the model group showed decreased food intake, lack of energy, sluggish activity, decreased urine output, weight loss and pathological changes of kidney tissue, whereas Irbesartan group and Tangshenping capsule group improved to some extent, The capsule group was superior to the irbesartan group. In the normal group, there was a small amount of MCP-1 protein and mRNA in the cytoplasm of renal tubular epithelial cells and renal interstitial cells. The model group showed strong positive expression. The expression of MCP-1 protein and mRNA in Tangshen Ping capsule group was significantly lower than that in model group (P <0.05). Conclusion: Tangshenping capsule may play a role in the treatment and prevention of diabetic nephropathy by down-regulating the expression of MCP-1 protein and mRNA in renal tissue.