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关东丁香(Syringa velutina)的茎皮在韩国用来治牙痛、肠道不适和腹泻。作者为了寻找抗癌的天然化合物,从关东丁香的茎皮中分离出多种化合物,经IR和NMR测定的数据分析,确定它们都是已知化合物,经体外癌细胞试验,首次发现其中两个化合物有抗癌活性。 阴干的关东丁香茎皮7.2kg,切碎后用甲醇回流提取3次,提取液减压浓缩成胶状(350g),将其混悬于水,依次用氯仿、乙酸乙酯、正丁醇提取。取一部分乙酸乙酯提取物用硅胶柱分离,用氯仿-甲醇-水洗脱,得到6个部分,部分1和2再用制备型TLC
The bark of Syringa velutina is used in South Korea to treat toothache, intestinal discomfort, and diarrhea. In order to search for natural compounds against cancer, the author isolated a variety of compounds from the bark of Guandong’s clove and analyzed them by IR and NMR to determine that they are all known compounds. The compound has anticancer activity. Dried Kanto clove bark 7.2kg, chopped and extracted with methanol reflux 3 times, the extract was concentrated under reduced pressure into a gel (350g), it was suspended in water, followed by extraction with chloroform, ethyl acetate, n-butanol . A portion of the ethyl acetate extract was separated on a silica gel column and eluted with chloroform-methanol-water to give six fractions. Fractions 1 and 2 were reconstituted with preparative TLC.