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目的 研究庚型肝炎病毒 (HGV)对恒河猴的致病性及其在恒河猴体内的复制和表达。方法 体外转录制备全长HGV基因组RNA ,肝内注射感染BY1猴 ,9个月后取其阳性血清感染BM 1猴 ,7个月后取BM 1阳性血清感染BB1。采用RT PCR、原位杂交、定量PCR和免疫学、血清酶学、组织病理学等方法 ,对HGV的感染性及致病性进行了研究。结果 BY1、BM1和BB1实验猴感染后 ,分别在第 3、8和 3周血清HGVRNA阳转 ,持续存在最长达 2 1周。血清丙氨酸转移酶 (ALT)均升高 ,最高达 418IU/L。感染后血清中均检测出抗 HGV。肝组织检查呈现轻度肝炎样变 ,并检测到HGVmRNA和HGVE2蛋白在肝组织中的表达。结论 体外转录的HGV基因组RNA对恒河猴具有感染性 ,可在恒河猴中传代感染并引起肝组织轻微炎症改变。恒河猴对HGV感染敏感 ,可作为研究HGV的实验动物模型
Objective To study the pathogenicity of hepatitis G virus (HGV) to rhesus monkeys and its replication and expression in rhesus monkeys. Methods The full length HGV genomic RNA was transcribed in vitro. BY1 monkey was infected by intrahepatic injection. Nine months after infection, BM1 monkeys were infected with the positive sera. After 7 months, BB1 was infected with BM1 positive sera. The infectivity and pathogenicity of HGV were studied by RT PCR, in situ hybridization, quantitative PCR and immunology, serum enzymology and histopathology. Results After BY1, BM1 and BB1 experimental monkeys were infected, the serum HGVRNAs were positive at weeks 3, 8 and 3, respectively, and persisted for up to 21 weeks. Serum alanine aminotransferase (ALT) were elevated up to 418IU / L. Anti-HGV was detected in the sera after infection. Liver tissue examination showed mild hepatitis-like changes, and the expression of HGV mRNA and HGVE2 protein in liver tissue was detected. Conclusions In vitro transcribed HGV genomic RNA is infectious to rhesus monkeys and can be passaged in rhesus monkeys and cause mild inflammatory changes in the liver tissue. Rhesus monkeys are sensitive to HGV infection and can be used as experimental animal models for studying HGV