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目的 :了解早期糖尿病肺形态学及氧化应激的变化。方法 :应用体视学方法对四氧嘧啶诱导的糖尿病 2 8d大鼠肺进行定量研究、观察超微结构变化并测定血清和肺组织的超氧化物歧化酶 (SOD)活性及丙二醛 (MDA)含量。结果 :糖尿病大鼠肺泡体密度和平均截线长度减少 ,肺泡壁体密度、肺泡表面积密度、肺泡数密度、肺泡面数密度和肺泡比表面增加。糖尿病大鼠Ⅱ型肺泡细胞粗面内质网扩张 ,其体密度、面密度、平均截面积和平均周长增加 ,而比表面减少。肺上皮和毛细血管基底膜增厚。糖尿病大鼠血清SOD活性下降 ,MDA含量增加 ;肺组织MDA含量明显升高。结论 :糖尿病早期大鼠肺已发生形态学变化且受到氧化应激的危害 ,提示肺脏是糖尿病损害的“靶器官”之一。
Objective: To understand the changes of lung morphology and oxidative stress in early stage of diabetes. Methods: The lungs of alloxan-induced diabetes mellitus (DM) -induced diabetic rats were quantitatively studied by stereological methods. The changes of ultrastructure were observed and the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and lung tissue were measured. )content. Results: The alveolar body density and mean length of line were decreased in diabetic rats. The alveolar wall density, alveolar surface area density, alveolar number density, alveolar number density and alveolar ratio increased. In type 2 diabetic alveolar cells, rough endoplasmic reticulum dilated, and its body density, areal density, average cross-sectional area and average perimeter increased, while the specific surface area decreased. Pulmonary epithelium and capillary basement membrane thickening. Diabetic rats serum SOD activity decreased MDA content increased; MDA content in lung tissue was significantly increased. CONCLUSION: The morphological changes of lungs and the damage of oxidative stress in the early stage of diabetic rats suggest that the lungs are one of the “target organs” for the damage of diabetes.