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目的：研究膀胱移行细胞癌中P16基因甲基化失活及其在肿瘤发生中的作用。方法：应用DNA甲基化分析技术检测52例膀胱移行细胞癌中P16基因5’CPG岛的甲基化状态，应用RT-PCR检测P16基因mRNA的表达。结果：52例膀胱移行细胞癌中19例出现P16基因5’CPG岛的甲基化，甲基化率为36.54%，而发生甲基化的肿瘤均未检测到P16mRNA表达。P16基因5’CPG岛甲基化多见于高期、高级肿瘤(P<0.05)。结论：P16基因5’CPG岛的甲基化引起的基因失活是膀胱肿瘤发展过程中的晚期所见。
Objective: To investigate the methylation inactivation of P16 gene in bladder transitional cell carcinoma and its role in tumorigenesis. Methods: Methylation status of P16 5’CPG island in 52 cases of BTCC was detected by DNA methylation analysis. The expression of P16 mRNA was detected by RT-PCR. Results: Methylation of 5’CPG island of P16 gene was detected in 19 cases of bladder transitional cell carcinoma, with a methylation rate of 36.54%. However, no methylation of P16 mRNA was detected in all of the 19 cases. P16 gene 5’CPG island methylation more common in advanced, advanced tumors (P <0.05). CONCLUSION: Gene inactivation caused by methylation of 5’CPG island of P16 gene is seen in the advanced stage of bladder tumor development.