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目的通过建立环境卷烟烟雾(environmental cigarette smoke,ETS)染毒模型,观察ETS对大鼠血脂水平、体内脂蛋白脂肪酶(LPL)和肝脂肪酶(HL)活力的影响。方法将20只健康清洁级SD大鼠随机分为吸烟组和对照组,每组10只,雌雄各半。吸烟组每天染毒2次,每次点燃卷烟6支,染毒60min,每周染毒6d,共染毒8周。测定大鼠血清中甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白(HDL)及低密度脂蛋白(LDL)的水平和血清、肝脏和肺脏中的LPL和HL活力。结果吸烟组与对照组大鼠体重间比较,差异无统计学意义。与对照组相比,吸烟组大鼠血清TG较高,HDL较低,差异均有统计学意义(P<0.05);TC与LDL也有升高趋势,差异均无统计学意义。吸烟组肝脏和肺脏的LPL、HL活力以及血清HL活力低于对照组,差异均有统计学意义(P<0.05)。结论 ETS可引起大鼠体内脂肪代谢酶活力降低,导致血脂代谢紊乱。
Objective To establish an environmental cigarette smoke (ETS) model to observe the effects of ETS on blood lipids, lipoprotein lipase (LPL) and hepatic lipase (HL) in rats. Methods Twenty healthy and clean Sprague-Dawley rats were randomly divided into smoking group and control group, with 10 mice in each group. The smoking group was given 2 times a day, 6 cigarettes were lit at a time, and the drug was exposed to 60 minutes of treatment for 6 days a week for a total of 8 weeks. Serum levels of triglyceride (TG), cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) and LPL and HL in serum, liver and lung were measured. Results There was no significant difference in body weight between smoking group and control group. Compared with the control group, the serum TG and HDL were lower in the smoking group than in the control group (P <0.05), while TC and LDL also increased. There was no significant difference between the two groups. The LPL, HL activity and serum HL activity of smoking group were lower than those of the control group (P <0.05). Conclusion ETS can cause the decrease of fat metabolism enzyme activity in rats, resulting in dyslipidemia.