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20只大白鼠分三组,即幼龄鼠组、成龄鼠组和老龄鼠组。用苏木素一伊红染色、尼氏染色,在光镜下观察;用吖啶橙 RNA 和 DNA 染色,在荧光显微镜下观察及用超薄切片在电镜下观察。组织学变化如下:比较三组动物的蓝斑细胞,在老化过程中呈现各种改变。在光镜下观察,随龄增长,蓝斑核的细胞密度减低。除去细胞缺失外,残存细胞显示缩小和尼氏体减少。若干细胞呈细胞固缩、核质溶解及胞质内 RNA 消失。围绕溃变细胞周围有卫星细胞增多。在电镜下观察,老化细胞显示异染色质增多和常染色质减少。细胞器亦显示若干变化,如核蛋白体减少,线粒体改变及脂褐素增多。这些老化改变对神经元蛋白质合成和功能方面可有严重影响。
Twenty rats were divided into three groups: young group, adult group and aging group. Stained with hematoxylin and eosin, observed with Nissl microscope and observed with light microscope; stained with acridine orange RNA and DNA, observed under a fluorescence microscope and observed with ultrathin sections under electron microscope. Histological changes are as follows: Comparison of three groups of animals, blue spot cells, showing a variety of changes in the aging process. Under light microscope observation, with age, the cell density of the locus coeruleus decreased. Except for cell loss, the remaining cells showed shrinkage and reduction of Nissl. Some cells showed cell shrinkage, cytoplasmic RNA and RNA disappeared. There are many satellite cells around the degenerative cells. Under electron microscopy, aged cells showed an increase in heterochromatin and a decrease in euchromatin. Organelles also show several changes, such as reduced ribosomes, altered mitochondria and increased lipofuscin. These aging changes can have serious effects on neuronal protein synthesis and function.