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目的观察乳杆菌DM9811发酵液提取物中RNA组分对人结肠癌细胞系HT-29增殖的影响,探讨其对肠道肿瘤细胞的作用及其分子机制,为阐明乳杆菌与宿主相互作用规律的分子机制奠定基础。方法应用MTT方法研究不同时间不同浓度RNA组分对HT-29增殖的影响,应用流式细胞术、RT-PCR研究RNA组分对HT-29细胞周期的影响。结果乳杆菌DM9811发酵液中RNA组分能抑制HT-29细胞增殖,并呈现出时间-剂量依赖性;RNA组分作用于HT-29细胞24 h、48 h时,细胞周期G0/G1期所占比例明显上升,S期所占比例明显降低(P<0.01),细胞周期调控因子CDK6、p27Kip1、p53的表达升高,CDK2、CDK4、PCNA的表达降低。结论乳杆菌DM9811代谢产物RNA在体外具有抑制癌细胞周期活性。
OBJECTIVE To observe the effect of RNA components in Lactobacillus DM9811 fermentation broth extract on the proliferation of human colon cancer cell line HT-29 and to explore its effect on intestinal tumor cells and its molecular mechanism. To elucidate the interaction between Lactobacillus and host Molecular mechanisms to lay the foundation. Methods MTT method was used to study the effects of different concentrations of RNA components on the proliferation of HT-29 cells. The effects of RNA components on the cell cycle of HT-29 cells were studied by flow cytometry and RT-PCR. Results The RNA fraction of Lactobacillus DM9811 could inhibit the proliferation of HT-29 cells in a time-and dose-dependent manner. After treated with RNA for 24 h and 48 h, the cell cycle of G0 / G1 phase (P <0.01). The expressions of CDK6, p27Kip1 and p53 were significantly increased and the expressions of CDK2, CDK4 and PCNA were decreased. Conclusion Lactobacillus DM9811 metabolite RNA can inhibit the cell cycle activity of cancer cells in vitro.