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目的:通过检测非小细胞肺癌组织及其癌旁组织中wip1、p38MAPK、p53表达水平,探讨非小细胞肺癌(non-small cell lung cancer)中wip1表达的临床意义。方法:用实时定量聚合酶链反应(quantitative realtime PCR)检测60例肺癌及其癌旁组织中wip1、p38MAPK、p53 mRNA表达,分析wip1 mRNA表达与临床病理之间的关系;Western blotting方法检测标本中wip1、p38MAPK、p53蛋白表达。结果:正常组和肿瘤组相比p38MAPK、p53的mRNA表达无差异。肿瘤组wip1 mRNA相对表达量为2.40±1.1,与正常组比较表达量明显升高,差异具有统计学意义(t=9.94,P<0.001)。wip1在肿瘤组和正常组中相对蛋白表达量分别为0.38±0.11、1.09±0.32,肿瘤组较正常组高,差异具有统计学意义(t=15.8,P<0.01)。正常组与肿瘤组比较,正常组p38MAPK及p53蛋白表达明显升高,差异均具有统计学意义(前者t=11.1,P<0.01;后者t=17.80,P<0.01)。wip1表达与肿瘤病理及分期有关,差异具有统计学意义(P<0.05)。结论:wip1在早期非小细胞肺癌中高表达,对p53、p38MAPK蛋白表达起负反馈抑制作用,初步研究结果提示wip1可能在非小细胞肺癌发生发展过程中起着重要的致癌作用。
OBJECTIVE: To investigate the expression of wip1, p38MAPK and p53 in non-small cell lung cancer (NSCLC) and its adjacent non-small cell lung cancer (NSCLC) and to investigate the clinical significance of wip1 expression in non-small cell lung cancer. Methods: The expressions of wip1, p38MAPK and p53 mRNA in 60 cases of lung cancer and its adjacent tissues were detected by quantitative realtime PCR. The relationship between wip1 mRNA expression and clinical pathology was analyzed. Western blotting was used to detect the expression of wip1, wip1, p38MAPK, p53 protein expression. Results: Compared with p38MAPK and p53, there was no difference in p53 mRNA expression between normal group and tumor group. The relative expression of wip1 mRNA in tumor group was 2.40 ± 1.1, which was significantly higher than that in normal group (t = 9.94, P <0.001). The relative protein expression of wip1 in tumor group and normal group were 0.38 ± 0.11 and 1.09 ± 0.32, respectively, which were significantly higher in tumor group than in normal group (t = 15.8, P <0.01). The expression of p38MAPK and p53 in normal group was significantly higher than that in normal group (P <0.01), while the difference was statistically significant (t = 11.1, P <0.01; The expression of wip1 was correlated with tumor pathology and staging, the difference was statistically significant (P <0.05). CONCLUSION: wip1 is overexpressed in early stage non-small cell lung cancer (NSCLC) and plays a negative feedback role in the expression of p53 and p38MAPK. Preliminary results suggest that wip1 may play an important role in the carcinogenesis and progression of NSCLC.