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目的通过观察新型植物灭螺剂HL对钉螺体内酶活性的影响,以期阐明HL类植物灭螺药物对钉螺的作用机理。方法将钉螺分别浸泡于25mg/L、50mg/L、100mg/L HL植物灭螺剂和去氯水中48h后,去壳、分离软体、置冰冻切片机切片,用酶组织化学方法显示乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、乙酰胆碱酯酶(AChE)、细胞色素C氧化酶(CCo)和一氧化氮合酶(NOS),观察其变化,并采用显微图像分析系统,通过比较灰度值定量测定其活性。结果钉螺LDH、SDH、AChE、CCo和NOS定位于肌纤维、口囊、表膜、神经节、消化腺、咽管等部位酶活性随浸泡HL灭螺剂浓度的增大而酶活性减弱越明显,且与对照组有显著差异。结论HL的灭螺作用机制可能是由于破坏钉螺体内神经效应与信息分子传递,以及抑制能量供应,最终引起钉螺死亡。其作用机制与氯硝柳胺相似。
Objective To observe the effect of HL, a new plant molluscicide, on the enzymatic activity of snail in order to clarify the mechanism of action of snail HL. Methods The snails were soaked in 25 mg / L, 50 mg / L and 100 mg / L HL molluscicidal agents and dechlorinated water for 48 h. The tissues were detached and the frozen section was sliced. The enzymatic histochemical method was used to study the dehydrogenation of lactic acid (LDH), succinate dehydrogenase (SDH), acetylcholinesterase (AChE), cytochrome C oxidase (CCo) and nitric oxide synthase (NOS) were measured and the changes were observed. The microscopic image analysis system , And its activity was quantitatively determined by comparing the grayscale values. Results The enzymatic activity of snails LDH, SDH, AChE, CCo and NOS localized in muscle fiber, capsule, epiretinal membrane, ganglion, digestive gland and pharynx were significantly decreased with the increase of concentration of soaking HL molluscicide, There was significant difference with the control group. Conclusion The mechanism of molluscicidal action in HL may be attributed to the destruction of snail nerve and the transmission of information molecules, as well as the suppression of energy supply, eventually resulting in the death of Oncomelania. Its mechanism of action and niclosamide similar.