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目的 研究通脉汤对氧化低密度脂蛋白 (oxLDL)损伤的人脐静脉内皮细胞产生一氧化氮 (NO)及纤溶平衡的影响。方法 制备通脉汤含药血清及oxLDL ,将人脐静脉内皮细胞ECV -3 0 4分为 4组 :⑴空白对照组 ;⑵胎牛血清对照组 ;⑶oxLDL损伤组 :oxLDL终浓度 10 0mg/L ;⑷通脉汤组 :含 5 %通脉汤血清及 10 0mg/LoxLDL ,孵育 2 4h后 ,倒置显微镜观察内皮细胞形态变化 ,测定上清液中NO、纤溶酶原激活物 (t -PA)及纤溶酶原激活物抑制物 -1(PAI -1)浓度。结果 ⑴oxLDL组细胞发生损伤改变 ,通脉汤组细胞形态明显好转 ;⑵oxLDL组NO及t -PA浓度低于空白对照组 (P <0 0 5 ) ,通脉汤组NO及t -PA浓度高于oxLDL损伤组 (P <0 0 5 ) ;⑶oxLDL组PAI -1浓度高于空白对照组 ( P <0 0 5 ) ,通脉汤组PAI -1浓度低于oxLDL组 ( P <0 0 5 )。结论 通脉汤含药血清可保护oxLDL损伤的人脐静脉内皮细胞 ,NO生成增加 ,恢复纤溶系统的平衡。
Objective To study the effect of Tongmai Decoction on the production of nitric oxide (NO) and fibrinolytic balance in human umbilical vein endothelial cells injured by oxidized low-density lipoprotein (oxLDL). Methods The serum containing Tongmai Decoction and oxLDL were prepared. The human umbilical vein endothelial cells ECV-304 were divided into 4 groups: (1) blank control group; (2) fetal bovine serum control group; (3) oxLDL injury group: oxLDL final concentration 100 mg/L (4) Tongmai decoction group: containing 5% Tongmai decoction serum and 100mg/LoxLDL, incubated for 24 hours, the morphology of endothelial cells was observed under an inverted microscope, and the supernatant was assayed for NO and plasminogen activator (t-PA); ) and plasminogen activator inhibitor-1 (PAI-1) concentrations. Results (1) The damage of cells in oxLDL group was changed, and the cell morphology of Tongmai Tang group was significantly improved; (2) The concentrations of NO and t-PA in oxLDL group were lower than those in blank control group (P < 0.05). The concentrations of NO and t-PA in Tongmai Tang group were higher than those in the control group (P <0.05). In the oxLDL injury group (P < 0.05), (3) The oxLDL group PAI-1 concentration was higher than the blank control group (P <0 05), and the Tongmai Decoction group PAI-1 concentration was lower than the oxLDL group (P <0 05). Conclusion Tongmai decoction containing serum can protect human umbilical vein endothelial cells damaged by oxLDL, increase NO production, and restore the balance of fibrinolysis system.