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目的观察普萘洛尔对体外培养的血管瘤内皮细胞凋亡的影响。方法取婴幼儿增殖期血管瘤标本,用组织块法原代培养血管瘤内皮细胞,并用第Ⅷ凝血因子相关抗原进行鉴定。待细胞处于对数生长期,换无血清培养基孵育48 h,使细胞同步化,然后分别加入0.5μmol·L-1和1.0μmol·L-1的普萘洛尔作为干预组,而对照组不加任何干预因素,继续孵育24 h,检测血管瘤血管内皮细胞凋亡率。结果血管瘤内皮细胞于组织块接种后3～4 d开始贴壁生长,内皮细胞呈多角形态,3～4周细胞开始快速生长,并铺满板底。培养细胞第Ⅷ凝血因子相关抗原表达为阳性。普萘洛尔作用24 h后,0.5μmol·L-1普萘洛尔组和1.0μmol·L-1普萘洛尔组血管瘤内皮细胞凋亡率分别为(22.42±0.83)%、(24.36±1.42)%,较对照组[(15.72±0.59)%]均明显升高(Pa<0.01)。结论普萘洛尔可以促进体外培养的血管瘤内皮细胞凋亡或抑制其增殖。
Objective To observe the effect of propranolol on the apoptosis of endothelial cells in vitro. Methods The specimens of proliferating hemangiomas from infants and young children were obtained. The endothelial cells of primary hemangioma were cultured by tissue block method and identified by the antigen of Ⅷ coagulation factor. When the cells were in logarithmic growth phase and incubated for 48 h in serum-free medium, the cells were synchronized and then added propranolol (0.5 μmol·L -1 and 1.0 μmol·L -1) as the intervention group, while the control group Without any intervention, continue to incubate 24 h, detect the rate of vascular endothelial cell apoptosis. Results The hemangioma endothelial cells began adherent growth 3 to 4 days after inoculation of the tissue pieces. The endothelial cells showed polygonal morphology. The cells started to grow rapidly after 3 to 4 weeks and covered the bottom of the plate. Cultured cells Ⅷ coagulation factor-related antigen was positive. After 24 hours of propranolol treatment, the apoptotic rates of endothelial cells of hemangiomas with 0.5 μmol·L -1 propranolol and 1.0 μmol·L -1 propranolol were (22.42 ± 0.83)% and (24.36% ± 1.42)%, which was significantly higher than that of the control group [(15.72 ± 0.59)%] (Pa0.01). Conclusion Propranolol can promote endothelial cell apoptosis or inhibit its proliferation in vitro.