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目的探讨胜利油田矿区家蝇抗药性现状和防制对策。方法捕蝇笼采集试虫,在实验室饲养1~2代进行点滴法生物测试,并测定蛋白质含量和相关酶的活性。结果油田矿区家蝇对溴氰菊酯、高效氯氰菊酯和氯菊酯具有高抗性,抗性系数(R/S)分别为22.86、24.12和19.37;对敌敌畏的抗性有衰退趋势。家蝇经高效氯氰菊酯抗性选育后,Ach E、CAT、GST的活性均明显增加,分别为9.012、10.012和421.35 U/mgprot。在无选择压力下,家蝇高效氯氰菊酯抗性种群会出现抗性自然衰退趋势。结论实施以环境治理为主的综合防制措施,定期开展家蝇抗药性监测,合理使用杀虫剂,抑制和延缓抗性发展。
Objective To explore the status quo and control strategies of Musca domestica resistance in Shengli Oilfield. Methods The fly catching cage was used to collect the test insects, and the animals were cultured in the laboratory for 1 ~ 2 generations for bioassay. The protein content and the activity of related enzymes were also determined. Results Houseflies in the oilfields had high resistance to deltamethrin, beta-cypermethrin and permethrin with resistance ratios (R / S) of 22.86, 24.12 and 19.37, respectively. The resistance to dichlorvos tended to decline. After the housefly was bred by beta-cypermethrin, the activities of Ach E, CAT and GST were significantly increased, which were 9.012, 10.012 and 421.35 U / mgprot, respectively. In the absence of selective pressure, the beta-cypermethrin-resistant population of flies will show a natural decline trend. Conclusion The implementation of integrated environmental control-based prevention and control measures to carry out regular monitoring of housefly drug resistance, rational use of pesticides to inhibit and delay the development of resistance.