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目的检测辅助性T淋巴细胞17(Th17)及细胞因子IL-17、IL-21在支气管哮喘(哮喘)患儿外周血中的表达,探讨其在儿童哮喘发病机制中的作用。方法以哮喘患儿60例(哮喘组)为研究对象,根据病情分为哮喘发作期(发作期组,n=30)、哮喘缓解期(缓解期组,n=30);以健康儿童30例作为健康对照组。分离外周血单个核细胞,采用流式细胞术检测其Th17细胞百分率。采用ELISA法检测各组IL-17、IL-21表达水平。采用SPSS 13.0软件进行统计学分析。结果哮喘发作期组患儿外周血中Th17及IL-17水平明显高于缓解期组和健康对照组(Pa<0.05);哮喘发作期组患儿血清IL-21表达水平明显低于缓解期组和健康对照组(Pa<0.05);哮喘缓解期组与健康对照组外周血Th17、IL-17、IL-21水平比较,差异均无统计学意义(Pa>0.05)。结论 Th17、IL-17通过全身系统性炎症反应参与儿童哮喘的发病,IL-21可作为儿童哮喘的控制指标,亦有望为哮喘的治疗提供新的靶点。
Objective To detect the expression of Th17 and IL-17 and IL-21 in peripheral blood of children with bronchial asthma (asthma) and to explore their roles in the pathogenesis of childhood asthma. Methods Sixty asthmatic children (asthma group) were divided into three groups: asthma attack group (n = 30), remission stage of asthma (remission group, n = 30) As a healthy control group. Peripheral blood mononuclear cells were isolated and the percentage of Th17 cells was detected by flow cytometry. The expression of IL-17 and IL-21 in each group was detected by ELISA. SPSS 13.0 software was used for statistical analysis. Results The levels of Th17 and IL-17 in children with asthma attack were significantly higher than those in remission and healthy controls (Pa, <0.05). The levels of IL-21 in children with asthma attack were significantly lower than those in remission (P <0.05). There was no significant difference in the levels of Th17, IL-17 and IL-21 between the remission group and the healthy control group (Pa> 0.05). Conclusion Th17 and IL-17 are involved in the pathogenesis of childhood asthma through systemic systemic inflammatory response. IL-21 may be used as a control indicator of childhood asthma and is also expected to provide a new target for the treatment of asthma.