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通过液体摇床振荡培养实验,获得了链孢囊菌降解纤维素形成的菌体,采用元素组成、差热分析和红外光谱法,初步研究其化学结构和碱提取组分碳的分配状况,旨在探索链孢囊菌降解纤维素后是否能形成腐殖质及其碱提取组分是否为腐殖质组分。结果表明:(1)链孢囊菌降解纤维素后形成的菌体各组分碳量的分配主要集中于“水溶性组分”与“碱溶酸不溶组分”(相当于类胡敏酸),“碱溶酸溶组分”(相当于类富里酸)最少,培养后期(38～60 d)各组分碳,随着培养时间的延长,先形成“碱溶酸溶组分”后转化为“碱溶酸不溶组分”的速率减慢;(2)接种链孢囊菌后,培养初期(7～28 d),菌体C含量、H/C、O+S/C、放热高/中比,均呈现不规律的动态变化,培养后期(38～60 d),随着培养时间的延长,菌体缩合程度升高,氧化程度降低,芳香性增强;(3)菌体(60 d)的H/C、O+S/C均高于黑土胡敏酸(HA),而菌体的C含量、放热高/中比均低于黑土胡敏酸(HA),且分子结构较黑土胡敏酸(HA)简单。
The bacterial cell formed by degradation of cellulose by cyst culture was obtained by shaking culture experiment with liquid shaker. The chemical composition and the distribution of carbon in alkali extraction component were studied preliminarily by elemental composition, differential thermal analysis and infrared spectroscopy. Whether humus can be formed after exploring the degradation of cellulose by cyst and whether its alkaline extraction component is humus component is explored. The results showed that: (1) The distribution of carbon content of each component of bacteria formed after the degradation of cellulose by cyst culture mainly concentrated in the “water-soluble component” and “alkali-soluble acid insoluble components” Type humic acid), “alkali-soluble acid-soluble component” (equivalent to the class of fulvic acid) the least, the late cultivation (38 ~ 60 d) of each component of carbon, with the extension of culture time, (2) After inoculation of Streptococcus sacchari, the content of C and H in the initial stage of culture (7 ~ 28 d) / C, O + S / C, exothermic high / medium ratio, all showed irregular dynamic changes. During the later stage of culture (38-60 d), the degree of condensation and the degree of oxidation decreased with the extension of culture time (3) The H / C, O + S / C of the bacteria (60 d) were higher than that of the black soil humic acid (HA), while the C content and exothermic high / middle ratio of the bacteria were all lower In black soil humic acid (HA), and the molecular structure of black soil humic acid (HA) is simple.