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建立了红参须中人参皂甙Rg1的高效液相色谱测定法。采用HypersilODS柱,以甲醇:水(54:46)为流动相,在203nm处检测,平均回收率为99.34%,相对标准偏差为0.51%。该方法准确、灵敏。
A high performance liquid chromatographic method for determination of ginsenoside Rg1 in red ginseng was established. Using Hypersil ODS column with methanol: water (54:46) as the mobile phase at 203nm, the average recovery was 99.34% and the relative standard deviation was 0.51%. The method is accurate and sensitive.