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目的 探讨葡萄糖转运蛋白 (GL UT)的表达在胎儿生长受限 (fetal growth restriction,FGR)发生发展中的作用以及补肾益气活血防治 FGR的分子机制。 方法 用被动吸烟法建立 FGR大鼠的动物模型 ,并分为正常对照组、FGR组、FGR中药治疗组 (中药组 )和 FGR左旋精氨酸治疗组(L- Arg组 ) ,利用 RT- PCR和 Western blot的方法分别检测各组孕鼠胎盘葡萄糖转运蛋白 (GL U T)家族中 GL U T1 、GL UT3的 m RNA含量和蛋白浓度 ,同时还观察胎鼠和母鼠血清葡萄糖水平。 结果 FGR胎鼠血清葡萄糖 (2 .7± 0 .9) mm ol/ L 较正常组 (5 .3± 1.5 ) m mol/ L 明显降低 (P<0 .0 1) ;胎鼠血清葡萄糖在正常组及中药组之间以及各组母体血清葡萄糖之间都无明显差异 (P>0 .0 5 ) ;FGR组胎盘中 GL UT1 和 GL U T3的 m RNA含量及蛋白浓度较正常组明显降低 ,经补肾益气活血方治疗 ,孕鼠胎盘中两者的含量同正常组比较无明显差异。 结论 GL U T1 和 GL UT3参与了 FGR的病理生理过程 ;补肾益气活血方通过增强 GL U T1 和 GL U T3的基因的表达 ,提高胎鼠血清葡萄糖水平来防治 FGR。
Objective To investigate the role of glucose transporter (GLUT) expression in the development of fetal growth restriction (FGR) and the molecular mechanism of Bushengyiqihuoxue in preventing and treating FGR. Methods The animal model of FGR rats was established by passive smoking method and divided into normal control group, FGR group, FGR traditional Chinese medicine group and L-arginine FGR group (L-Arg group) And Western blot were used to detect the mRNA and protein concentrations of GLU T1 and GLUT3 in the GLUTs of the pregnant rats in each group. Serum glucose levels were also observed in the fetal and maternal rats. Results FGR fetal rat serum glucose (2.70 ± 0.9) mm ol / L was significantly lower than the normal group (5.3 ± 1.5) m mol / L (P <0.01); fetal rat serum glucose There was no significant difference between the Chinese herbal medicine group and the maternal serum glucose (P> 0.05). The content of m RNA and the protein concentration of GLUT1 and GLU T3 in the placenta of FGR group were significantly lower than those in the normal group After the treatment of invigorating the kidney and invigorating the qi and invigorating the blood, the contents of both in the placenta of pregnant rats have no significant difference compared with the normal group. Conclusion GL U T1 and GL UT3 are involved in the pathophysiology of FGR. Bushen Yiqi Huoxue Decoction can prevent and treat FGR by enhancing the expression of GL U T1 and GL U T3 genes and raising the level of serum glucose in fetus.