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Objective: The objective of this study was to investigate whether 70％ aqueous ethanol extract of San?Jia?Fu?Mai decoction extract (SJFMDE) could protect against 1?methyl?4?phenylpyridinium (MPP+)?induced oxidative stress in PC12 cells and 1?methyl?4?phenyl?1,2,3,6?tetrahydropyridine (MPTP)?induced motor function deficits in mice. Materials and Methods: The cell viability, the levels of intracellular reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione (GSH) in the MPP+?treated PC12 cells were measured. Motor function deficits and dopamine (DA) level in the brain striatum and tyrosine hydroxylase (TH)?positive cells in substantia nigra pars compacta (SNc) of the MPTP?treated mice were determined. Results: The results showed that SJFMDE could reduce cell death and the levels of ROS and MDA while increase the level of GSH in the MPP+?treated PC12 cells. In addition, in vivo studies showed that oral administration of SJFMDE (3, 6, and 12 g/kg) significantly improved the motor function deficits induced by MPTP and enhanced the DA level in the striatum and TH?positive neuronal cells in SNc of the MPTP?treated mice. Conclusions: Our results revealed that SJFMDE possessed neuroprotective effects against neurotoxicity induced by MPP + and motor function deficits induced by MPTP via suppressing oxidative stress and increasing the levels of DA and TH, indicating that SJFMDE might be a promising Chinese medicine formula for the treatment of Parkinson's disease.