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目的构建临床代表性好、稳定性佳的艰难梭菌感染小鼠模型,为艰难梭菌感染相关疾病提供研究工具。方法选择C57BL/6、BALB/c、昆明小鼠,经抗生素诱导后,分别予以不同浓度(108 CFU/mL~10~(10) CFU/mL)的临床分离菌株混悬液灌胃,观察不同品系小鼠不同时间点腹泻、全身情况及结肠组织病理学变化。结果灌菌后,BALB/c小鼠10~(10) CFU/mL浓度组全部出现腹泻,死亡率为16.7%;其他实验组小鼠腹泻程度差异较大或仅部分出现轻度腹泻。腹泻小鼠表现为稀烂便甚至水样便和湿尾现象,体重减轻,肠道病理显示结肠黏膜充血水肿伴炎性细胞浸润。结论经5种抗生素灌胃9d+克林霉素腹腔注射诱导,10~(10) CFU/mL艰难梭菌活菌混悬液灌胃后构建的BALB/c小鼠艰难梭菌感染模型最稳定。
Objective To construct a mouse model of C. difficile infection with good clinical representativeness and stability and provide a research tool for diseases related to Clostridium difficile infection. Methods C57BL / 6, BALB / c and Kunming mice were inoculated with suspension of clinical isolates of different concentrations (108 CFU / mL ~ 10 ~ (10) CFU / mL) Diarrhea, systemic conditions and pathological changes of colon tissue of mice in different time points. Results All the mice in the 10 ~ (10) CFU / mL BALB / c mice group had diarrhea with a mortality rate of 16.7%. Other mice in the experimental group had more diarrhea or only mild diarrhea. Diarrhea mice showed a sparse or even watery stool and wet tail phenomenon, weight loss, intestinal pathology showed colonic mucosal congestion and edema with inflammatory cell infiltration. Conclusions The model of C. difficile infection in BALB / c mice induced by intraperitoneal injection of 5 kinds of antibiotics 9d + clindamycin intraperitoneally with 10 ~ (10) CFU / mL viable C. difficile suspension was the most stable.