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目的研究槲皮素对ultraviolet B(UVB)辐射引起的皮肤损伤的保护作用并探讨其可能的作用机制。方法分别建立小鼠和永生化的人角质形成细胞HaCaT的UVB损伤模型,研究体内和体外模型中槲皮素对受到紫外损伤的皮肤的保护作用。结果①经形态学观察,发现槲皮素能够显著缓解由UVB照射引起的皮肤水肿和炎症的发生。②动物组织切片免疫组织化学染色和细胞实时荧光定量PCR及Western blotting实验结果显示,UVB照射能够显著地提高皮肤组织和HaCaT细胞中COX-2的表达,而槲皮素能够在基因和蛋白水平显著地抑制UVB照射引起的COX-2的上调表达。结论槲皮素可能通过抑制诱导型环氧化酶COX-2的表达进而抑制UVB辐射引起的皮肤损伤。
Objective To study the protective effect of quercetin on skin lesions caused by ultraviolet B (UVB) radiation and to explore its possible mechanism. Methods UVB injury models of mouse and immortalized human keratinocytes HaCaT were established respectively to study the protective effect of quercetin on the skin damaged by UV light in vitro and in vivo. Results ① Morphological observation showed that quercetin could significantly alleviate the skin edema and inflammation caused by UVB irradiation. ② Immunohistochemical staining and real-time quantitative PCR and Western blotting results showed that UVB irradiation could significantly increase the expression of COX-2 in skin and HaCaT cells, and quercetin had a significant effect on the gene and protein levels Inhibited the upregulation of COX-2 induced by UVB irradiation. Conclusion Quercetin may inhibit the skin damage induced by UVB radiation by inhibiting the expression of COX-2, an inducible cyclooxygenase.