[Objective] To determine the types and contents of amino acids in the flowers of Fritilaria thunbergii Miq., so as to provide scientific basis for its further development.[Method] Natural dried, lyophilized and fluidized bed treated flowers of Fritillaria thunbergii Miq. were as test materials, and the content of amino acids in which were determined by ninhydrin column derivatization method. [Result] There were 17 types of amino acids in the flower of Fritillaria thunbergii Miq., and the total contents of amino acids in different treated Fritillaria thunbergii Miq.flowers achieved 200 g/kg, among which the content of glutamic acid was the highest, and the essential amino acids accounted for more than 30% in total.[ Conclusion] The flowers of Fritillaria thunbergii Miq.were endowed with high medical and healthy value, which deserve further research. [Objective] To determine the types and contents of amino acids in the flowers of Fritilaria thunbergii Miq., so as to provide scientific basis for its further development.[Method] Natural dried, lyophilized and fluidized bed treated flowers of Fritillaria thunbergii Miq. were As test materials, and the content of amino acids in which were determined by ninhydrin column derivatization method. [Result] There were 17 types of amino acids in the flower of Fritillaria thunbergii Miq., and the total contents of amino acids in different treated Fritillaria Thunbergii Miq.flowers achieved 200 g/kg, among which the content of glutamic acid was the highest, and the essential amino acids accounted for more than 30% in total.[ Conclusion] The flowers of Fritillaria thunbergii Miq.were endowed with high medical And healthy value, which deserve further research.