近年来,一些实验室多采用外周血、骨髓和睾丸等为材料来制备SCE,尚未见到利用淋巴器官内的淋巴细胞来制备SCE的报道。小鼠是生物学、医学等学科研究中最常用的实验动物之一,作者利用初生小鼠的脾脏细胞体外培养制备SCE,取得了成功。生后3—5天的杂种小白鼠幼鼠,经体表消毒后剖腹取脾脏。除去结缔组织,用Hank’s In recent years, some laboratories mostly use peripheral blood, bone marrow and testis for the preparation of SCE materials, have not yet seen the use of lymphocytes in lymphoid organs to prepare SCE reports. Mice are one of the most commonly used experimental animals in biology, medicine and other disciplines. The authors made use of spleen cells of newborn mice to prepare SCE in vitro and achieved success. 3-5 days after birth, hybrid mouse pups, the body surface after disinfection to take the spleen. Remove the connective tissue with Hank’s