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采集大港油田油井well-1017采出液,利用GC-MS分析油藏原油结构并利用16S r DNA克隆文库技术分析油藏内源微生物群落结构特征,探索极端油藏环境下细菌和古细菌的群落功能。研究结果表明:长期水驱开采导致了油藏采出液含水率、原油重质组分升高,原油品位下降。同时,油藏内源微生物16S r DNA克隆文库系统反映了油藏细菌群落包括Proteobacteria、Firmicutes、Nitrospirae、Bacteroidetes、Thermotogae、Deferribacteres、Caldiserica、Dictyoglomi和Aquificae等9个门类,而古细菌群落主要包括广古菌门Euryarchaeota的Methanobacteria、Archaeoglobi、Methanomicrobia和Thermococci等4个纲结构;微生物文库构成显示极端油藏环境下仍存在着丰富的功能微生物资源,其在石油烃降解、产表面活性剂和产甲烷等方面展现出较大的应用潜力。研究极端油藏微生物群落结构多样性,可以为微生物驱油技术在低品位油藏资源的开采应用中提供重要的生物信息。
The well-1017 produced oil in well of Dagang Oilfield was collected. The crude oil structure of the reservoir was analyzed by GC-MS. The structure of endogenous microbial community in the reservoir was analyzed by 16S rDNA cloning library to explore the community of bacteria and archaea in extreme reservoir environment Features. The results show that the long-term waterflooding results in the moisture content of the oil recovery fluid, the heavy components of the crude oil increase, and the crude oil grade decreases. At the same time, the 16S rDNA clone library system of reservoir endogenous microorganisms reflects that the reservoir bacterial community includes 9 categories, including Proteobacteria, Firmicutes, Nitrospirae, Bacteroidetes, Thermotogae, Deferribacteres, Caldiserica, Dictyoglomi and Aquificae. Methanobacteria, Archaeoglobi, Methanomicrobia and Thermococci in Euryarchaeota. The composition of the microbial library shows that there are still abundant functional microbial resources in extreme oil reservoirs. In terms of petroleum hydrocarbon degradation, surfactant production and methanogenesis Show great potential for application. Studying the diversity of microbial community structure in extreme reservoirs can provide important biological information for microbial flooding technology in the exploitation of low-grade reservoir resources.