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目的分析2009年龙岩市流行的人肠道病毒71(EV71)分离株的分子生物学特征。方法从手足口病例的153份咽拭标本中分离EV71病毒株,选取其中3株,用RT-PCR扩增VP1基因片段,并进行测序和病毒种系进化等分析。结果 3株EV71均为C4基因亚型,与其他省份C4亚型分离株的同源性为94.8%～99.0%;其中EV71/FJ-LY241/09和EV71/FJLY299/09具有更高的同源性,而另1株EV71/FJLY231/09与其他2株距离稍远。氨基酸序列分析表明,3株病毒的VP1具有3个氨基酸位点差异。结论 2009年龙岩市流行的EV71为C4基因亚型,与目前国内其他省份流行的毒株无显著差别,但不同分离株有一定差异,其原因需进一步监测和研究。
Objective To analyze the molecular biological characteristics of the human enterovirus 71 (EV71) isolated in 2009 in Longyan City. Methods The EV71 strain was isolated from 153 pharyngeal swab specimens from 3 cases of hand-foot-mouth disease and 3 strains were selected. The VP1 gene fragment was amplified by RT-PCR and sequenced. Results The three strains of EV71 were all C4 gene subtypes with 94.8% -99.0% homology with other strains of C4 subtype in other provinces. EV71 / FJ-LY241 / 09 and EV71 / FJLY299 / 09 had higher homology While the other strain, EV71 / FJLY231 / 09, was slightly apart from the other two strains. Amino acid sequence analysis showed that three strains of VP1 have three amino acid differences. Conclusion The prevalence of EV71 in 2009 in Longyan is C4 subtype, which is not significantly different from other strains present in other provinces in China. However, there are some differences among the isolates. The reasons for this need further monitoring and research.