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目的:分析异鼠李素-3-O-β-D-芸香糖苷经体外培养大鼠肠道菌群代谢转化的产物。方法:在离体培养的大鼠肠道菌群中,加入异鼠李素-3-O-β-D-芸香糖苷,采用HPLC检测代谢进程,采用超高效液相色谱串联电喷雾飞行时间质谱(UPLC-ESI-Q-TOF-MS-MS)对转化产物进行分析,结合对照品、化合物精确分子量和CID-MS-MS裂解碎片信息进行化合物结构解析。结果:从异鼠李素-3-O-β-D-芸香糖苷的大鼠肠道代谢产物中鉴定了槲皮素-3-O-葡萄糖苷、异鼠李素-3-O-葡萄糖苷、槲皮素、山奈酚和异鼠李素,另一化合物是否为5,7,3’,4’-四甲氧基黄酮-3-O-葡萄糖苷还需进一步确证。结论:肠道菌群代谢对异鼠李素-3-O-β-D-芸香糖苷的转化主要是苷水解、脱羟基和甲基化,增加了化合物疏水性和化学多样性。
OBJECTIVE: To analyze the metabolic transformation of isorhamnetin-3-O-β-D-rutinoside in rat intestinal flora. METHODS: The isorhamnetin-3-O-β-D-rutinoside was isolated from the intestinal tract of rat in vitro. The metabolic processes were determined by HPLC. The compounds were characterized by ultra performance liquid chromatography-tandem electrospray ionization time of flight mass spectrometry (UPLC-ESI-Q-TOF-MS-MS) to analyze the conversion products, combined with the reference substance, the precise molecular weight of the compound and CID-MS-MS fragmentation information for compound structure analysis. Results: Quercetin -3-O-glucoside, isorhamnetin-3-O-glucoside were identified from rat intestinal metabolites of isorhamnetin-3-O-β-D-rutinoside , Quercetin, kaempferol and isorhamnetin, and whether the other compound is 5,7,3 ’, 4’-tetramethoxyflavone-3-O-glucoside needs further confirmation. CONCLUSION: The metabolism of isorhamnetin-3-O-β-D-rutinoside in intestinal microflora is mainly caused by glycoside hydrolysis, dehydroxylation and methylation, which increase the hydrophobicity and chemical diversity of the compounds.