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磷是植物生长发育所需的大量营养元素之一,当周围环境中磷缺乏时,植物往往通过扩大根系范围来增加对土壤中磷的吸收,同时调节一些生化代谢途径,增加磷酸酶、有机酸等物质的分泌从而活化土壤中固定的难溶性磷.本研究利用水稻全基因组寡核苷酸芯片对水稻中早18分别在正常营养条件和低磷胁迫处理条件下6,24,72h3个时间点的根部和地上部材料进行基因表达谱分析.研究结果共鉴定出低磷胁迫差异表达基因1207个,其中根部差异表达基因795个,地上部差异表达基因450个,根部和地上部共同出现的差异表达基因38个.功能分析表明,这些差异表达基因包括了代谢调节离子转运、信号传导、转录调节、和逆境应答等方面的基因.同时发现水稻在低磷胁迫后大量转座子基因在转录水平上发生了变化.这些研究结果为进一步揭示植物磷代谢调控机理的研究提供了有用的信息.
Phosphorus is one of the macronutrients required for plant growth and development. When phosphorus is scarce in the surrounding environment, plants often increase phosphorus uptake in the soil by expanding the root range, and at the same time, regulate some biochemical metabolic pathways and increase the amount of phosphatase and organic acids And other substances in order to activate fixed insoluble phosphorus in soil.In this study, rice genome-wide oligonucleotide microarray in early 18 were normal nutrient conditions and phosphorus stress treatment conditions 6,24,72 h 3 time points Of the roots and shoots were analyzed by gene expression profiling.Results A total of 1207 differentially expressed genes were identified under low-phosphorus stress, of which 795 differentially expressed genes at root and 450 differentially expressed genes at shoots, and the common differences between root and shoot Expression of genes 38. Functional analysis showed that these differentially expressed genes include genes that regulate ion transport, signal transduction, transcriptional regulation, and stress response, etc. At the same time, we found that a large number of transposon genes were transcribed The results of these studies to further reveal the mechanism of plant phosphorus metabolism regulation provides a useful Information.