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香蕉SSR分子标记开发和应用有限,通过对棉花SSR分子标记在香蕉中的通用性研究。用1343对棉花EST-SSR引物对贡蕉(AA)和野蕉(BB)两个材料进行转移扩增,得到226对有效扩增的引物。进一步用该226对引物对20个香蕉品种和4个野蕉材料扩增,得到了157对多态性引物。157对多态性引物共扩增到1188个条带,平均7.6个;引物的多态信息含量范围在0.58～0.91之间。依据获得的SSR数据,应用非加权类平均聚类方法,在相似系数0.60水平上,将24个品种分为两大类群,类群I是以A基因组为主的品种群,类群Ⅱ是以B基因组和A基因组与B基因组杂交的品种群,证明了棉花EST-SSR分子标记在香蕉中具有通用性。
Banana SSR molecular marker development and application of limited, through the cotton SSR molecular markers in banana in the common research. 1343 cotton EST-SSR primer pairs of banana (AA) and wild banana (BB) two materials were amplified, amplified 226 pairs of primers for effective amplification. The 226 pairs of primers were further amplified from 20 banana varieties and 4 wild banana materials, 157 pairs of polymorphic primers were obtained. 157 pairs of polymorphic primers were amplified to 1188 bands, an average of 7.6; primers polymorphic information content range between 0.58 ~ 0.91. Based on the SSR data obtained, non-weighted average clustering method was used to classify 24 cultivars into two groups based on the similarity coefficient of 0.60. Group I was a group of A-dominant breeds, and group II was based on the B genome And breed populations of A and B genomes proved that cotton EST-SSR molecular markers are common in banana.