用溶剂蒸馏法制备了马鞭草醇提液 ( EV) ,分别作用于体外培养的绒毛膜癌 JAR细胞、人肝癌SMMC-772 1细胞及人胚肺二倍体成纤维细胞 ( 2 BS)。MTT法及荧光法检测结果表明 ,EV对 JAR细胞增殖有明显抑制作用 ,两种方法测得抑制率分别为 71 .9%± 4 .0 %和 69.6%± 2 .0 % ;同时还测得 EV对 JAR细胞质中表皮生长因子受体 ( EGFR)的表达也有明显抑制作用 ,含 2 5mg/ ml和 50 mg/ ml EV的培养液对其抑制率分别为 3 6.7%和 80 .6%。而 EV对 SMMC-772 1细胞及 2 BS细胞则无明显影响。EV对体外培养的绒毛膜癌 JAR细胞有明显抑制作用 ,且具有特异性 ,这一作用可能与抑制 EGFR的表达有关。 Verbena liquid extract (EV) was prepared by solvent distillation and was applied to cultured choriocarcinoma JAR cells, human hepatocellular carcinoma SMMC-772 1 cells and human embryonic lung diploid fibroblasts (2 BS) respectively. The results of MTT assay and fluorescence assay showed that EV significantly inhibited the proliferation of JAR cells. The inhibition rates of the two methods were 71.9% ± 4.0% and 69.6% ± 2.0%, respectively. At the same time, EV also significantly inhibited the expression of epidermal growth factor receptor (EGFR) in JAR cytoplasm. The inhibition rates of EV with 25 mg / ml and 50 mg / ml EV were 37.6% and 80.6%, respectively. However, EV had no significant effect on SMMC-772 1 cells and 2 BS cells. EV can significantly inhibit the JAR cells cultured in vitro and has specificity, which may be related to the inhibition of the expression of EGFR.