山杨(Populus davidiana Dode)是我国分布最广,面积最大,生长迅速、材质优良,宜于山地生长的森林更新先锋树种。分析研究山杨染色体数目和染色体组型,是寻找发现我国天然三倍体山杨,研究其遗传变异的可靠途径。目前,国内常用树木染色体制片方法是浮尔根,铁矾—苏木精、醋酸洋红染色等,但对山杨染色体染色效果不理想,计数较为困难。因为,我们采 Populus davidiana Dode is the most widely distributed, the largest area, rapid growth, material quality, suitable for mountain regeneration of the pioneer tree species. It is a reliable way to find out the natural triploid populus asiaticus in China and to study its genetic variation by analyzing the chromosome number and chromosome constitution of Populus euphratica. At present, the commonly used method of tree chromosome preparation in the country is floating root, iron alum - hematoxylin, acetic acid magenta staining and so on, but the staining effect on Aspen is not ideal and counting is more difficult. Because, we adopt