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目的 研究山莨菪碱 (65 4 2 )对创伤性急性肺损伤 (ALI)家兔血浆中肿瘤坏死因子 α(TNF α)、白细胞介素 6(IL 6)的含量及其在内脏组织中mRNA表达的影响。方法 用ELISA和RT PCR方法测定 2 4只大耳白兔血浆中TNF α、IL 6的含量及其在内脏组织中mRNA的表达。结果 创伤性ALI家兔与正常组比较 ,血浆中TNF α、IL 6的含量升高 (P <0 .0 5 ) ,内脏组织中TNF α、IL 6mRNA的表达显著增多 (P <0 .0 1)。65 4 2治疗后明显降低ALI家兔血浆中TNF α、IL 6的含量 (P<0 .0 5 ) ,抑制内脏组织TNF α、IL 6mRNA的表达 (P <0 .0 5 )。结论 65 4 2能有效抑制ALI家兔多种组织TNF α、IL 6mRNA表达 ,降低血浆中TNF α、IL 6的含量。
Objective To investigate the effects of anisodamine (65 4 2) on the levels of tumor necrosis factor α (TNF α), interleukin 6 (IL 6) and its mRNA expression in visceral tissues of rabbits with traumatic acute lung injury (ALI) Impact. Methods The contents of TNFα and IL-6 in plasma and the expression of mRNA in visceral tissues of 24 rabbits were determined by ELISA and RT-PCR. Results Compared with the normal control group, the levels of TNFα and IL-6 in plasma of traumatic ALI rabbits increased (P <0.05), while the levels of TNFα and IL-6 mRNA in visceral tissues increased significantly (P <0.01) ). After treatment, the levels of TNFα and IL-6 in plasma of ALI rabbits were significantly decreased (P <0.05), and the expressions of TNFα and IL-6 mRNA in visceral tissues were inhibited (P <0.05). Conclusion 65 4 2 can effectively inhibit the expression of TNFα and IL-6 mRNA in various tissues of ALI rabbits and decrease the contents of TNFα and IL-6 in plasma.