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目的建立定量测定多种丹参酚酸的高效液相色谱(HPLC)分析方法,考察丹参素和原儿茶醛对丹酚酸B降解规律的影响。方法应用HPLC技术对丹酚酸B降解样品中的4种酚酸进行定量分析,应用LC-MS技术剖析样品的化学物质基础,对加入丹参素和原儿茶醛前后的丹酚酸B样品进行研究。结果丹酚酸B降解产物主要有9种,包括丹参素、原儿茶醛、丹酚酸B的多种异构体、紫草酸以及多种紫草酸异构体。加入相同质量浓度的丹参素和原儿茶醛,原儿茶醛对丹酚酸B降解的保护作用更明显,紫草酸异构体I和II的峰面积随着初始原儿茶醛质量浓度的增大而有所增加。结论原儿茶醛对丹酚酸B降解的保护作用可能是依赖于其弱酸性,紫草酸异构体I和II的峰面积受原儿茶醛初始质量浓度的影响可能与反应平衡改变有关。
OBJECTIVE To establish a HPLC method for the quantitative determination of Salvia miltiorrhiza Bunge, and investigate the effects of Danshensu and protocatechuic aldehyde on the degradation of Salvianolic acid B. Methods Four kinds of phenolic acids in salvianolic acid B degradation samples were quantitatively analyzed by HPLC. The chemical bases of samples were analyzed by LC-MS. The samples of salvianolic acid B before and after the addition of danshensu and protocatechuic aldehyde the study. Results There were 9 main metabolites of salvianolic acid B, including Danshensu, protocatechuic aldehyde, multiple isomers of salvianolic acid B, lithoxan, and many kinds of isomers of lithoxanthin. Adding the same mass concentration of Danshensu and protocatechuic aldehyde, protocatechuic aldehyde has a more obvious protective effect on the degradation of salvianolic acid B. The peak areas of the isolates I and II of the lithoxanic acid with the initial protocatechuic aldehyde mass concentration Increased and increased. Conclusion Protocatechuic aldehyde may have a protective effect on the degradation of salvianolic acid B, which may be due to its weak acidity. The peak area of I and II may be influenced by the initial equilibrium concentration of protocatechuic aldehyde.