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目的:探讨四味肝泰纳米颗粒对黄曲霉毒素B1诱发大鼠肝癌模型的药效作用及其作用机理。方法:采用血清常规肝功检测、抗氧化实验、Bax/Bcl-2和TNF-α免疫组织化学分析以及HE染色方法对黄曲霉毒素B1诱发大鼠肝癌模型进行检测。结果:各剂量四味肝泰纳米颗粒(2.03,1.01,0.5 mg.kg-1,ig)均可有效降低大鼠肝脏损伤程度,并能通过提高抗氧化酶表达来降低过氧化脂质水解物丙二醛含量;同时可以促使Bax过量表达,导致大鼠体内TNF-α含量升高,促使癌细胞凋亡,从而发挥抗肝癌作用。结论:四味肝泰纳米颗粒对黄曲霉毒素B1诱发的大鼠肝癌具有显著疗效,这与其抗氧化作用有关。
Objective: To investigate the pharmacodynamic effects and the mechanism of Siwei Ganteng nano-particles on aflatoxin B1-induced hepatocellular carcinoma in rats. Methods: Aflatoxin B1 -induced hepatocellular carcinoma (HCC) rat model was tested by serum routine liver function test, antioxidant test, Bax / Bcl-2 and TNF-α immunohistochemistry and HE staining. Results: Each dose of Siwei Gan Tai nano-particles (2.03,1.01,0.5 mg.kg-1, ig) can effectively reduce the degree of liver damage in rats, and can improve antioxidant enzyme expression to reduce peroxide lipid hydrolyzate Malondialdehyde content; at the same time can promote Bax overexpression, resulting in rat TNF-α content increased, to promote cancer cell apoptosis, which play a role in anti-liver cancer. CONCLUSION: Siwei Gantel nanoparticle has a significant effect on aflatoxin B1-induced hepatocellular carcinoma in rats, which is related to its antioxidative effect.