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目的:观察CETP转基因小鼠在卵巢切除和雌激素替代情况下糖耐量和胰岛功能的改变。方法:15只雌性CETP转基因小鼠随机分为3组,假手术组、卵巢切除组和雌激素替代组。除假手术组外,其余均行卵巢切除术。术后第2周开始,雌激素替代组给予雌激素治疗,而假手术组和卵巢切除组则给予同等剂量的橄榄油,4周后检测各组小鼠空腹血糖,8周后进行腹腔注射葡萄糖耐量(IPGTT)试验,提取小鼠胰岛做葡萄糖刺激的胰岛素分泌(GSIS)实验。结果:术后8周,卵巢切除组小鼠空腹血糖较假手术组鼠明显升高(P<0.05),IPGTT各时点血糖均较假手术组小鼠明显升高(P<0.05),GSIS显示胰岛素分泌功能有减退的趋势;而雌激素替代组上述变化有明显改善,IPGTT的0、15、30 min的血糖水平显著下降(P<0.05),GSIS的胰岛素分泌功能也有改善的趋势。结论:CETP转基因小鼠卵巢切除后胰岛功能下降,表现为空腹血糖升高、糖耐量受损、葡萄糖刺激的胰岛素分泌水平下降。雌激素替代能够对卵巢切除所带来的对于胰岛β细胞的有害影响起到一定的保护作用。
Objective: To observe the changes of glucose tolerance and pancreatic islet function in CETP transgenic mice under ovariectomy and estrogen replacement. Methods: Fifteen female CETP transgenic mice were randomly divided into 3 groups: sham operation group, ovariectomized group and estrogen replacement group. In addition to the sham group, the others underwent ovariectomy. From the second week after operation, the estrogen replacement group was given estrogen treatment, while the sham operation group and the ovariectomy group were given the same dose of olive oil. After four weeks, the fasting blood glucose of the mice in each group was tested. After 8 weeks, Tolerance (IPGTT) assay was used to extract mouse islets glucose-stimulated insulin secretion (GSIS). Results: After 8 weeks of operation, the fasting blood glucose in ovariectomized mice was significantly higher than that in sham-operated mice (P <0.05). The blood glucose of IPGTT mice at each time point was significantly higher than that in sham-operated mice (P <0.05) (P <0.05). The insulin secretion function of GSIS also showed a tendency of improvement. However, the above changes in estrogen replacement group were significantly improved. The blood glucose levels of IPGTT at 0, 15 and 30 min decreased significantly (P <0.05). CONCLUSION: The islet function of CETP transgenic mice after ovariectomy is decreased. The fasting blood glucose, impaired glucose tolerance and glucose-stimulated insulin secretion are decreased. Estrogen replacement can play a protective role in the detrimental effects of ovariectomy on pancreatic β-cells.