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目的观察活血化瘀中药与高压氧对脑缺血再灌注小鼠血小板膜糖蛋白、白细胞黏附分子表达的影响。方法采用暂时结扎双侧颈总动脉的方法制造脑缺血再灌注模型,实验共分7组:假手术组、缺血再灌注组(模型组)、高压氧组、联合治疗大剂量组、联合治疗中剂量组、联合治疗小剂量组、中药中剂量组。高压氧处理组在造模后第1天开始进行高压氧处理;药物处理组在造模前5 d开始按规定剂量经胃灌服中药,每天1次;各组分别在造模后1、3、5 d 3个不同时间点处死小鼠,从小鼠眶动脉和眶静脉采血1 mL,采用流式细胞术观察各组小鼠各时点血小板膜糖蛋白CD31、CD61、CD62p及白细胞黏附分子CD11a/CD18、CD11b/CD18、CD62L的变化。结果①活血化瘀中药联合高压氧可有效抑制脑缺血再灌注后血小板膜糖蛋白CD31、CD61、CD62p及白细胞黏附分子CD11a/CD18、CD11b/CD18、CD62L的异常表达。从3种用药剂量对检测指标的作用效果来看,以活血化瘀中药中剂量组与高压氧联合应用效果更好。②单纯活血化瘀中药中剂量组亦可明显抑制脑缺血再灌注后血小板膜糖蛋白CD31、CD61、CD62p及白细胞黏附分子CD11a/CD18、CD11b/CD18、CD62L的异常表达。结论活血化瘀中药对脑缺血再灌注损伤具有一定的保护作用。
Objective To observe the effects of traditional Chinese medicine and hyperbaric oxygen on the expression of platelet membrane glycoprotein and leukocyte adhesion molecules in mice with cerebral ischemia-reperfusion. Methods The model of cerebral ischemia / reperfusion was established by temporary ligation of bilateral common carotid arteries. The experiment was divided into 7 groups: sham operation group, ischemia reperfusion group (model group), hyperbaric oxygen group, combined treatment of high dose group Middle-dose group, low-dose combination therapy group and medium-dose Chinese medicine group. The hyperbaric oxygen treatment group started hyperbaric oxygen treatment on the first day after the model was established. The drug treatment group began to take traditional Chinese medicine via stomach once a day at 5 d before the model making, , And mice were sacrificed at 3 different time points. Blood samples were collected from orbital artery and orbital vein of mice by flow cytometry. The expressions of platelet membrane glycoprotein CD31, CD61, CD62p and leukocyte adhesion molecule CD11a / CD18, CD11b / CD18, CD62L changes. Results ①Huangxing Huayu combined with hyperbaric oxygen could effectively inhibit the abnormal expression of platelet membrane glycoproteins CD31, CD61, CD62p and leukocyte adhesion molecules CD11a / CD18, CD11b / CD18 and CD62L after cerebral ischemia / reperfusion. From the effect of three kinds of dosage on the detection index, the effect of combined application of hyperbaric oxygen and middle-dose group of traditional Chinese medicine for activating blood circulation and removing blood stasis is better. ② The middle-dose of traditional Chinese medicine alone can obviously inhibit the abnormal expression of platelet membrane glycoprotein CD31, CD61, CD62p and leukocyte adhesion molecules CD11a / CD18, CD11b / CD18 and CD62L after cerebral ischemia / reperfusion. Conclusion Chinese Herbs for Activating Blood Circulation and Removing Blood Stasis has a protective effect on cerebral ischemia-reperfusion injury.