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目的探讨树突状细胞在新疆维吾尔族妇女宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及宫颈癌中的检测及意义。方法 将HLA-DR、CD1a、S-100蛋白作为DC特异性标记物,采用免疫组织化学方法对20例慢性宫颈炎、70例CIN(CINⅠ25例、CINⅡ25例、CINⅢ20例)以及40例浸润性宫颈鳞癌组织标本中DC的分布和表达进行检测。结果 S-100、CD1a和HLA-DR阳性表达率依次为浸润性宫颈鳞癌(65.00%,55.00%,62.50%)>CIN(50.00%,44.29%,40.00%)>慢性宫颈炎(30.00%,20.00%,25.00%),差异具有统计学意义(P=0.035,P=0.037,P=0.012),S-100、CD1a和HLA-DR分布于癌组织及癌巢附近的间质中。在宫颈各组织中,CD1a与S-100蛋白表达呈正相关(r=0.330,P=0.000);CD1a与HLA-DR表达也呈正相关(r=0.267,P=0.002);而S-100蛋白与HLA-DR表达没有相关性(r=0.065,P=0.461)。结论人宫颈上皮内瘤变至浸润癌变过程中树突状细胞特异性标记物S-100、HLA-DR、CD1a表达强度发生变化,提示宫颈病变局部的免疫功能发生了改变。
Objective To investigate the significance of dendritic cells in cervical intraepithelial neoplasia (CIN) and cervical cancer in Uighur women in Xinjiang. Methods 20 cases of chronic cervicitis, 70 cases of CIN (25 cases of CINⅠ, 25 cases of CINⅡ, 20 cases of CINⅢ) and 40 cases of infiltrating cervix were enrolled in this study. HLA-DR, CD1a and S-100 proteins were used as DC specific markers. The distribution and expression of DC in squamous cell carcinoma specimens were detected. Results The positive rates of S-100, CD1a and HLA-DR in invasive cervical squamous cell carcinoma were 65%, 55.00% and 62.50%, respectively. CIN (50.00%, 44.29%, 40.00% 20.00%, 25.00%). The difference was statistically significant (P = 0.035, P = 0.037, P = 0.012). The distribution of S-100, CD1a and HLA-DR was located in the interstitial of cancer tissue and cancer nests. There was a positive correlation between the expression of CD1a and S-100 protein in cervical tissues (r = 0.330, P = 0.000). There was also a positive correlation between CD1a and HLA-DR expression (r = 0.267, P = 0.002) HLA-DR expression was not correlated (r = 0.065, P = 0.461). Conclusion The expression level of dendritic cell-specific markers S-100, HLA-DR and CD1a in human cervical intraepithelial neoplasia changed to invasive carcinogenesis, suggesting that local immunologic function of cervical lesions changed.