论文部分内容阅读
分别灌胃给予川芎哚和［２－１５Ｎ］川哚后，尿样经酶水解、碱化、有机溶剂提取。有机相（碱性和中性部分）经浓缩并硅烷化；水相经酸化、吹干提取（提取物为酸性水溶部分）并硅烷化。用ＧＣ－ＭＳ测定。在碱性和中性提取物中，检出原型川穹哚及其一种代谢物；在酸性水溶性提取物中，检出川芎哚的另两种代谢物。结果表明，川芎哚体内代谢途径可能是川芎哚羟基化和川芎哚羟甲基的氧化。
After oral gavage of Chuanxiong and [2-15N] -cholesterole respectively, the urine samples were extracted by enzymatic hydrolysis, alkalization and organic solvent. The organic phase (basic and neutral) is concentrated and silanized; the aqueous phase is acidified, air-dried for extraction (the extract is an acidic water-soluble fraction) and silanized. Measured by GC-MS. In alkaline and neutral extracts, a prototype of Chuan dome vale and one of its metabolites was detected; in the acidic water-soluble extract, the other two metabolites of Chuanxiongole were detected. The results showed that the metabolic pathway of Rhizoma Chuanxiongdan could be the hydroxylation of Chuanxiong and hydroxymethyl of Chuanxiong.