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为筛选防治牡丹黑斑病的化学药剂,采用菌丝生长速率法和涂布平板法分别测定了多菌灵、苯醚甲环唑和嘧菌酯对牡丹黑斑病菌菌丝生长的抑制活性及对分生孢子萌发和形成的影响。结果表明:苯醚甲环唑对菌丝生长的抑制作用最强,有效中浓度EC50仅为0.30μg/mL,其次为嘧菌酯6.34μg/mL和多菌灵92.18μg/mL;多菌灵可显著降低分生孢子的萌发速率,而苯醚甲环唑和嘧菌酯对孢子萌发的抑制作用较弱,可使分生孢子及芽管发生畸形,并改变分生孢子的萌发方式;3种药剂均可显著抑制芽管的伸长生长,推迟病菌分生孢子梗的形成时间,但对分生孢子的产生数量无显著影响。多菌灵可作为保护剂在病害发生前期使用,苯醚甲环唑和嘧菌酯可在病害发生期轮换使用。
In order to select the chemical agents for preventing and curing peony black spot, the mycelial growth rate method and the coating plate method were respectively used to determine the inhibitory activity of carbendazim, difenoconazole and azoxystrobin on mycelial growth of Alternaria pebina, Effect on conidial germination and formation. The results showed that difenoconazole had the strongest inhibitory effect on mycelial growth with EC50 of only 0.30μg / mL, followed by azoxystrobin 6.34μg / mL and carbendazim 92.18μg / mL. Carbendazim Can significantly reduce the conidia germination rate, and difenoconazole and azoxystrobin inhibition of spore germination is weak, can conidia and germ tube deformity, and change the germination of conidia; 3 All kinds of pesticides could significantly inhibit the elongation growth of germ tube, postponed the formation of pathogen of conidiophores, but had no significant effect on the number of conidia. Carbendazim can be used as a protective agent in the pre-disease stage, difenoconazole and azoxystrobin can be used during the rotation of the disease.