The complete mitochondrial genome of Ampittia dioscorides (Lepidoptera:Hesperiidae) was determined.The sequenced genome is a circular molecule of 15313 bp,containing 13 protein-coding genes (PCGs),22 tRNA genes,two rRNA genes,and an A + T-rich region.The gene arrangements and transcribing directions are identical to those in most of the reported lepidopteran mitogenomes.The base composition of the whole genome and genes or regions are also similar to those in other lepidopteran species.All the PCGs are initiated by typical ATN codons;the exception being COⅠ,which begins with a CGA codon.Eight genes (ND2,ATPase8,ATPase6,COⅢ,ND5,ND4L,ND6,and Cytb) end with a TAA stop codon,and two genes (ND1 andND3) end with TAG.The remaining three genes (COⅠand COⅡ,which end with TA-,and ND4,which ends with T-) have incomplete stop codons.All tRNAs have the typical clover-leaf structure of mitochondrial tRNAs,with the exception of tRNASer(AGY).On the basis of the concatenated nucleotide and amino acid sequences of the 13 PCGs and wingless gene of 22 butterfly species,maximum parsimony (MP) and Bayesian inference (BI) trees were constructed,respectively.Both MP and BI trees had the same topological structure:((((Nymphalidae + Danaidae) + Lycaenidae) + Pieridae) + Papilionidae) + Hesperiidae).The results provide support for Hesperiidae as a superfamily-level taxon.