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目的利用离子交联和化学交联相结合的方法制备壳聚糖纳米粒子(NPs),并对NPs分别进行了叶酸(FA)和聚乙二醇(PEG)的修饰。方法通过红外光谱进行结构验证;用扫描电镜和粒度分析仪对粒子的微观形态、粒径、电位等进行了表征;通过与Hela细胞摄取实验对其靶向作用进行验证。结果离子交联和化学交联相结合的方法制备壳聚糖纳米粒子粒径在200 nm左右并且粒径分布窄,修饰后的NPs(FA-NPs、PEG-NPs及FA+PEG-NPs)粒径不受功能基团修饰的影响。激光共聚焦试验证明FA-NPs及FA+PEG-NPs能显著提高细胞对粒子的摄取,而PEG-NPs则明显降低其对粒子的摄取。结论 FA+PEG-NPs有望成为一种新型的药物载体,用于抗癌药物对癌细胞的主动靶向。
OBJECTIVE To prepare chitosan nanoparticles (NPs) by a combination of ionic crosslinking and chemical crosslinking, and to modify NPs with folic acid (FA) and polyethylene glycol (PEG) respectively. Methods The structure was verified by FTIR. The morphology, particle size and potential of the particles were characterized by scanning electron microscopy and particle size analyzer. The targeting effects were verified by Hela cell uptake assay. Results The combination of ionic crosslinking and chemical crosslinking was used to prepare chitosan nanoparticles with a diameter of about 200 nm and a narrow particle size distribution. The modified NPs (FA-NPs, PEG-NPs and FA + PEG-NPs) Path is not affected by functional group modification. Confocal laser scanning microscopy demonstrated that FA-NPs and FA + PEG-NPs significantly increased cell uptake, whereas PEG-NPs significantly reduced uptake of the particles. Conclusion FA + PEG-NPs are expected to become a new type of drug carrier for the active targeting of cancer cells by anticancer drugs.