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０．０１—０．１ｍｍｏｌ／Ｌ腐胺Ｐｕｔｒｅｓｃｉｎｅ（Ｐｕｔ）浸种处理４８ｈ，可以促进水稻种子（加粒２２２）在次适温下（１６±１℃）萌发，显著地提高发芽指数和活力指数。但在２８℃时对种子萌发无显著影响。进一步研究表明，在次适温下（１６±１℃），Ｐｕｔ可以促进α－淀粉酶合成，提高α－淀粉酶活性。增加呼吸强度和ＡＴＰ含量。但在２８℃时，Ｐｕｔ对种子内α－淀粉酶活性无影响。研究结果表明，Ｐｕｔ对种子萌发的影响与温度有关，在次适温下（１６±１℃）可促进水稻种子（加粒２２２）萌发。
The soaking of 0.01-0.1 mmol / L Putrescine Put for 48 h promoted the germination of rice seed (plus granule 222) at sub-optimal temperature (16 ± 1 ℃), and significantly improved the germination index and vigor index. However, there was no significant effect on seed germination at 28 ℃. Further studies showed that under the mild temperature (16 ± 1 ℃), Put can promote the synthesis of α-amylase and improve the activity of α-amylase. Increase respiratory intensity and ATP content. But at 28 ℃, Put had no effect on the α-amylase activity in seeds. The results showed that the effect of Put on the seed germination was related to the temperature, and the seed germination was promoted at the suitable temperature (16 ± 1 ℃).