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目的:探讨肿节风总黄酮(ZJF-HT)对小鼠骨髓巨核系细胞扩增的影响。方法:采用液体培养和半固体集落培养,观察ZJF-HT及其含药血清对成熟巨核细胞及巨核系祖细胞集落扩增的作用。结果:与模型组相比,ZJF-HT≥125μg.mL-1能使成熟巨核细胞数和巨核系祖细胞集落数增多(P<0.05);ZJF-HT含药血清在0.39%~6.25%体积浓度范围能增加成熟巨核细胞数,在0.20%~6.25%范围内能显著增加巨核系祖细胞集落数(P<0.01)。结论:ZJF-HT及其含药血清均能促进巨核系细胞的体外扩增。
Objective: To investigate the effect of ZJF-HT on the proliferation of murine bone marrow megakaryocytes. Methods: Liquid culture and semi-solid colony culture were used to observe the effect of ZJF-HT and its serum containing on the proliferation of mature megakaryocytes and megakaryocyte progenitor cells. Results: Compared with the model group, ZJF-HT≥125μg.mL-1 could increase the number of mature megakaryocytes and the number of megakaryocyte progenitor cells (P <0.05); the concentration of ZJF-HT containing serum increased from 0.39% to 6.25% Concentration range can increase the number of mature megakaryocytes, in the range of 0.20% ~ 6.25% can significantly increase the number of megakaryocyte progenitor cells (P <0.01). Conclusion: Both ZJF-HT and its drug-containing serum can promote the proliferation of megakaryocytes in vitro.