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目的研究玉米赤霉烯酮(ZEA)和氯化镉(CdCl2)联合作用对MCF-7细胞增殖的影响。方法调整MCF-7细胞密度约为1×10~5/mL,以0(对照)、10~(-8)、10~(-7)、10~(-6)、10~(-5)、10~(-4)、10~(-3)mol/L CdCl_2分别染毒MCF-7细胞24h,以0(对照)、6.25、12.5、25、50、100nmol/L ZEA分别染毒MCF-7细胞24h,采用CCK-8法检测CdCl_2和ZEA对MCF-7细胞增殖的影响,同时进行3×3析因设计研究CdCl_2和ZEA对MCF-7细胞增殖的联合作用。结果 CdCl_2和ZEA分别在10~(-6) mol/L、100nmol/L时促增殖效应均达到最大,相对增殖率分别为112.3%和114.6%,明显高于阴性对照组的100.0%;析因实验结果显示CdCl_2和ZEA对MCF-7细胞增殖的影响表现为相加作用。结论 CdCl_2和ZEA在一定剂量范围内能促进MCF-7细胞增殖,表现为明显的拟雌激素活性,二者联合作用表现为加和效用。
Objective To study the effect of ZEA and CdCl2 on the proliferation of MCF-7 cells. Methods The density of MCF-7 cells was adjusted to about 1 × 10 ~ 5 / mL. The cells were treated with 0 (control), 10 -8, 10 -7, 10 -6, 10 -5, MCF-7 cells were exposed to 10 ~ (-4), 10 ~ (-3) mol / L CdCl_2 for 24 h respectively. The cells were exposed to ZEA 0, 6.25,12.5,25,50,100 nmol / 7 cells for 24 hours. The effects of CdCl_2 and ZEA on the proliferation of MCF-7 cells were detected by CCK-8 assay. The combined effects of CdCl_2 and ZEA on the proliferation of MCF-7 cells were also investigated by 3 × 3 factorial design. Results The proliferative effects of CdCl_2 and ZEA at the concentrations of 10 -6 mol / L and 100 nmol / L, respectively, reached the maximum with relative proliferation rates of 112.3% and 114.6%, respectively, which were significantly higher than those of the negative control group The experimental results show that the effects of CdCl 2 and ZEA on the proliferation of MCF-7 cells show an additive effect. Conclusion CdCl_2 and ZEA can promote the proliferation of MCF-7 cells in a certain dose range, showing obvious estrogenic activity. The combined effect of CdCl_2 and ZEA is additive and effective.