Breeding program strategies to develop novel short grain white rice varieties such as japonica(short grain)that introgress biotic stress resistance and high grain quality have been developed using indica rice(Pin Kaset+4 and Riceberry)for applications in japonica rice(Koshihikari)improvement.Four breeding lines showing promising agronomic performance with short grain and low amylose content(＜20％)were obtained.In addition,sensory testing of these breeding lines showed high scores that similar to Koshihikari.Two promising lines,KP48-1-5 and KP48-1-9,which possessed a combination of four genes resistance to different biotic stresses(Bph3+TPS+Xa21+Pi-ta)and four genes for grain quality(GS3+SSIIa+wxb+badh2),were developed using marker-assisted selection(MAS)with the pedigree method.The current study clearly illustrated the successful use of MAS in combining resistance to multiple biotic stresses while maintaining a high yield potential and preferred grain quality.Moreover,the results indicated that this breeding program,which includes crossing temperate japonica with indica,can create novel short grain rice varieties adapted to a tropical environment,like the japonica type.