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目的 探讨ATP诱导人白血病细胞U937细胞凋亡的机理。 方法 应用ATP(0 2 3g L)作用于培养的U937细胞 ,应用免疫荧光细胞化学方法检测连接蛋白Cx4 3、F 肌动蛋白 (F actin)、纽蛋白 (vinculin)的表达。 结果 ATP诱导U937细胞凋亡小体形成的同时Cx4 3表达增强、F 肌动蛋白重组 ,纽蛋白组装改善。 结论 ATP诱导人白血病细胞凋亡时 ,凋亡小体的形成与间隙连接蛋白Cx4 3表达、F 肌动蛋白重组 ,纽蛋白组装有着平行关系 ,表明它们在U937凋亡小体形成过程所起的重要作用
Objective To investigate the mechanism of ATP-induced apoptosis in human leukemia cell line U937. Methods The cultured U937 cells were treated with ATP (0 2 3 g L), and the expression of connexin Cx4 3, F actin and vinculin were detected by immunofluorescence cytochemistry. Results ATP induced the formation of apoptotic bodies in U937 cells while enhancing the expression of Cx4 3, F actin recombination, and improved assembly of anella protein. Conclusions ATP-induced apoptosis of human leukemia cells leads to the formation of apoptotic bodies in parallel with the expression of connexin Cx4 3, F-actin and vinculin, indicating that they are involved in the formation of U937 apoptotic bodies Important role