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对Ｃ１２～Ｃ２０等脂肪酸在反相高效液相色谱（ＨＰＬＣ）系统中的超微量分析进行了研究。月桂酸、肉豆蔻酸、棕榈酸、硬脂酸、花生酸等有机酸在所选择的色谱条件下都能得到有效的分离，采用Ｃ１８键合相的色谱柱，流动相采用甲醇∶水＝９７∶３，流速为１．５ｍＬ／ｍｉｎ，荧光检测波长为λｅｘ＝３２５ｎｍ，λｅｍ＝３９７ｎｍ。在实验中采用４－溴甲基－７－甲氧基香豆素作为Ｃ１２～Ｃ２０等脂肪酸的荧光标记物，以丙酮为溶剂，以１８－冠醚－６为催化剂，以碳酸钾为碱试剂，通过相转移催化完成衍生化反应。对方法的准确度、精密度及最小检出限进行了验证，最小检出限达到１０－７ｇ／Ｌ数量级
The ultramicro analysis of fatty acids such as C12-C20 in reversed-phase high performance liquid chromatography (HPLC) was studied. Organic acids such as lauric acid, myristic acid, palmitic acid, stearic acid, and arachidic acid can be effectively separated under the selected chromatographic conditions using a C18 bonded phase column with a mobile phase of methanol: water = 97 : 3, flow rate 1.5mL / min, fluorescence detection wavelength λex = 325nm, λem = 397nm. In the experiment, 4-bromomethyl-7-methoxycoumarin was used as a fluorescent label for fatty acids such as C12-C20, with acetone as solvent, 18-crown-6 as catalyst and potassium carbonate as base reagent , Through the phase transfer catalysis to complete the derivatization reaction. The method accuracy, precision and minimum detection limit were verified, the minimum detection limit of 10-7g / L order of magnitude