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目的:探讨增强夏枯草种子抗旱性的有效途径。方法:以不同浓度的聚乙二醇(PEG)溶液模拟干旱胁迫;引发处理后种子置于25%PEG干旱胁迫下发芽。结果:随着PEG胁迫浓度增加,夏枯草种子发芽率、发芽指数、活力指数下降;经过20%~25%PEG,300~500 mg.L-1GA3,1.60%~2.00%KNO3-KH2PO4引发处理,3个居群夏枯草种子在干旱胁迫下种子发芽率以及发芽指数显著提高;经过0.40%~1.20%NaCl引发处理,南京紫金山、安徽金寨夏枯草种子抗旱性显著增强,种子发芽率以及发芽指数显著提高;0.40%~2.00%NaCl引发处理,甘肃夏枯草种子发芽率、发芽指数降低。结论:适量浓度PEG,GA3,KNO3-KH2PO4引发,可以增强夏枯草种子萌发阶段抗旱能力。
Objective: To explore effective ways to enhance drought resistance of Prunella vulgaris seeds. Methods: Drought stress was simulated by different concentrations of polyethylene glycol (PEG) solution. The seeds were germinated under the drought stress of 25% PEG. Results: The germination rate, germination index and vigor index of Prunella vulgaris decreased with the increase of PEG stress concentration. After 20% -25% PEG, 300-500 mg · L-1 GA3, 1.60% -2.00% KNO3- The seed germination rate and germination index of three populations of Prunella vulgaris seedlings under drought stress increased significantly. After 0.40% ~ 1.20% NaCl treatment, seedling drought resistance of Prunella vulgaris in Nanjing Zijin Mountain and Anhui walled Jinzhai significantly increased, and seed germination rate and germination The index increased significantly. Germination rate and germination index of Prunella vulgaris seeds decreased with 0.40% ~ 2.00% NaCl treatment. Conclusion: The appropriate concentration of PEG, GA3, KNO3-KH2PO4 primed, can enhance the seed germination of Prunella drought resistance.