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卵裂间隔(τ0)是与温度相关的参数,为早期胚胎发育时期第一次卵裂持续的时间,或连续两次细胞分裂的时间间隔,被广泛用于鱼卵染色体操作中最佳诱导时间的确定上。本文研究了史氏鲟♀×达氏鳇♂受精卵在14～22℃下卵裂间隔τ0与温度的关系,τ0为第一次卵裂(τⅠ)与第二次卵裂(τⅡ)间的时间间隔(τⅡ-τⅠ)。结果表明:在实验温度范围内,史氏鲟♀×达氏鳇♂受精卵τ0随着温度(T)的上升而减小,在14℃下τ0为85.0±1.3 min,22℃下τ0为45.8±2.2 min,两者之间符合线性回归关系:τ0=154.54-4.95T(R2=0.999 0)。22℃为史氏鲟♀×达氏鳇♂胚胎发育的温度上限,超过此温度,正常卵裂的胚胎比例将显著下降(<60%)。
Cleavage interval (τ0) is a temperature-dependent parameter, which is the duration of the first cleavage in early embryonic development or the interval of two consecutive cell divisions. It is widely used in the optimal induction time for chromosome manipulation of fish eggs Ok on it. In this paper, the relationship between the cleavage interval τ0 and the temperature at 14-22 ℃ was studied. The relationship between τ0 and the temperature at the first cleavage (τⅠ) and the second cleavage (τⅡ) Time interval (τ Ⅱ-τ Ⅰ). The results showed that in the experimental temperature range, the τ0 of S. sturgeon × S. dahuricus ♂ decreased with increasing temperature (T), τ0 was 85.0 ± 1.3 min at 14 ℃, and τ0 was 45.8 at 22 ℃ ± 2.2 min, the linear regression relationship between the two: τ0 = 154.54-4.95T (R2 = 0.999 0). 22 ℃ is the upper temperature limit of Sturgeon ♀ × D ♂ ♂ ♂ embryo, beyond which the proportion of embryos with normal cleavage will decrease significantly (<60%).